DNMT3A ELISA Kit for Human(Homo sapiens) | EIAab.comDNMT3A ELISA Kit for Human(Homo sapiens)

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DNMT3A (Gene name),

DNA (cytosine-5)-methyltransferase 3A (Protein name ), DNM3A_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

DNMT3A;

Protein name:

DNA (cytosine-5)-methyltransferase 3A(Dnmt3a);

Alternative:

DNA methyltransferase HsaIIIA(DNA MTase HsaIIIA;M.HsaIIIA);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with UBC9, PIAS1 and PIAS2 (By similarity). Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD domain (By similarity). Interacts with DNMT1 and DNMT3B. Interacts with the PRC2/EED-EZH2 complex.

Function:

Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.

Subcellular Location:

Nucleus Cytoplasm Accumulates in the major satellite repeats at pericentric heterochromatin.

Protein Attributes:

Sequence length:

912

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

850:

900:

912:

LFAPLKEYFA | CV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

hsa:1788

MIM:

602769

STRING:

9606.ENSP00000264709

UniGene:

Hs.515840

SMR:

Q9Y6K1

Pfam:

PF00145

String:

Q9Y6K1

Uniprot:

Q9Y6K1

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ELISA Kit for Human Dnmt3a

Cat.:

E13889h

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Packing:

96T

ELISA Kit for Human Dnmt3a

Cat.:

E13889r

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96T

ELISA Kit for Human Dnmt3a

Cat.:

E13889m

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96T

ELISA Kit for Human Dnmt3a

Cat.:

E13889c

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96T

CLIA Kit for Human Dnmt3a

Cat.:

U13889m

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Packing:

96T

CLIA Kit for Human Dnmt3a

Cat.:

U13889r

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MSDS:

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Packing:

96T

CLIA Kit for Human Dnmt3a

Cat.:

U13889h

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MSDS:

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Packing:

96T

CLIA Kit for Human Dnmt3a

Cat.:

U13889c

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96T

Polyclonal Antibody for Human Dnmt3a

Cat.:

P13889Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human Dnmt3a

Cat.:

P13889Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human Dnmt3a

Cat.:

P13889Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human Dnmt3a

Protein for Human Dnmt3a

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Cloning, expression and chromosome locations of the human DNMT3 gene family."
Xie S. , Wang Z. , Okano M. , Nogami M. , Li Y. , He W.-W. , Okumura K. , Li E.
Gene236:87-95(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Fetal testis.
2.

"A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation."
Chen T. , Ueda Y. , Xie S. , Li E.
J. Biol. Chem.277:38746-38754(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
3.

"Co-operation and communication between the human maintenance and de novo DNA (cytosine-5) methyltransferases."
Kim G.-D. , Ni J. , Kelesoglu N. , Roberts R.J. , Pradhan S.
EMBO J.21:4183-4195(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);INTERACTION WITH DNMT1 AND DNMT3B;SUBCELLULAR LOCATION
4.

"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W. , Graves T.A. , Fulton R.S. , Fulton L.A. , Pepin K.H. , Minx P. , Wagner-McPherson C. , Layman D. , Wylie K. , Sekhon M. , Becker M.C. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , Nash W.E. , Kremitzki C. , Oddy L. , Du H. , more...
Nature434:724-731(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3)
tissue: Lung.
tissue: PNS.
tissue: Skin.
7.

"The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors."
Robertson K.D. , Uzvolgyi E. , Liang G. , Talmadge C. , Sumegi J. , Gonzales F.A. , Jones P.A.
Nucleic Acids Res.27:2291-2298(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
8.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
9.

"The histone methyltransferase SETDB1 and the DNA methyltransferase DNMT3A interact directly and localize to promoters silenced in cancer cells."
Li H. , Rauch T. , Chen Z.-X. , Szabo P.E. , Riggs A.D. , Pfeifer G.P.
J. Biol. Chem.281:19489-19500(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SETDB1
10.

"The Polycomb group protein EZH2 directly controls DNA methylation."
Vire E. , Brenner C. , Deplus R. , Blanchon L. , Fraga M. , Didelot C. , Morey L. , Van Eynde A. , Bernard D. , Vanderwinden J.-M. , Bollen M. , Esteller M. , Di Croce L. , de Launoit Y. , Fuks F.
Nature439:871-874(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH THE PRC2/EED-EZH2 COMPLEX
11.

Vire E. , Brenner C. , Deplus R. , Blanchon L. , Fraga M. , Didelot C. , Morey L. , Van Eynde A. , Bernard D. , Vanderwinden J.-M. , Bollen M. , Esteller M. , Di Croce L. , de Launoit Y. , Fuks F.
Nature446:824-824(2006)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
12.

"Polycomb-mediated methylation on Lys27 of histone H3 pre-marks genes for de novo methylation in cancer."
Schlesinger Y. , Straussman R. , Keshet I. , Farkash S. , Hecht M. , Zimmerman J. , Eden E. , Yakhini Z. , Ben-Shushan E. , Reubinoff B.E. , Bergman Y. , Simon I. , Cedar H.
Nat. Genet.39:232-236(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DE NOVO DNA METHYLATION OF TARGET GENES
13.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
14.

"Methyl-H3K9-binding protein MPP8 mediates E-cadherin gene silencing and promotes tumour cell motility and invasion."
Kokura K. , Sun L. , Bedford M.T. , Fang J.
EMBO J.29:3673-3687(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MPHOSPH8
15.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-243;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
16.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105; SER-243; SER-255; THR-261 AND SER-267;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
17.

"Mutations in the DNA methyltransferase gene DNMT3A cause an overgrowth syndrome with intellectual disability."
Childhood Overgrowth Consortium , Tatton-Brown K. , Seal S. , Ruark E. , Harmer J. , Ramsay E. , Del Vecchio Duarte S. , Zachariou A. , Hanks S. , O'Brien E. , Aksglaede L. , Baralle D. , Dabir T. , Gener B. , Goudie D. , Homfray T. , Kumar A. , Pilz D.T. , more...
Nat. Genet.46:385-388(2014) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN TBRS;VARIANTS TBRS ASN-310; SER-532; LYS-548; ARG-549; PRO-648; LEU-700; CYS-749; ASP-838; SER-902 AND LEU-904
18.

"Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation."
Jia D. , Jurkowska R.Z. , Zhang X. , Jeltsch A. , Cheng X.
Nature449:248-251(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.89 ANGSTROMS) OF 627-909 IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE;SUBUNIT;MUTAGENESIS OF PHE-732
19.

"Structural basis for recognition of H3K4 methylation status by the DNA methyltransferase 3A ATRX-DNMT3-DNMT3L domain."
Otani J. , Nankumo T. , Arita K. , Inamoto S. , Ariyoshi M. , Shirakawa M.
EMBO Rep.10:1235-1241(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.29 ANGSTROMS) OF 476-614 IN COMPLEXES WITH ZINC AND WITH HISTONE H3 PEPTIDE;SUBUNIT
20.

"MPP8 mediates the interactions between DNA methyltransferase Dnmt3a and H3K9 methyltransferase GLP/G9a."
Chang Y. , Sun L. , Kokura K. , Horton J.R. , Fukuda M. , Espejo A. , Izumi V. , Koomen J.M. , Bedford M.T. , Zhang X. , Shinkai Y. , Fang J. , Cheng X.
Nat. Commun.2:533-533(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) OF 40-53 IN COMPLEX WITH MPHOSPH8;INTERACTION WITH MPHOSPH8
21.

"Structural and histone binding ability characterizations of human PWWP domains."
Wu H. , Zeng H. , Lam R. , Tempel W. , Amaya M.F. , Xu C. , Dombrovski L. , Qiu W. , Wang Y. , Min J.
PLoS ONE6:E18919-E18919(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 275-427
22.

"Mutational spectrum analysis of chronic myelomonocytic leukemia includes genes associated with epigenetic regulation: UTX, EZH2, and DNMT3A."
Jankowska A.M. , Makishima H. , Tiu R.V. , Szpurka H. , Huang Y. , Traina F. , Visconte V. , Sugimoto Y. , Prince C. , O'Keefe C. , Hsi E.D. , List A. , Sekeres M.A. , Rao A. , McDevitt M.A. , Maciejewski J.P.
Blood118:3932-3941(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS ASP-699; PHE-731 DEL; CYS-882; HIS-882 AND PRO-882

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